Dna extraction protocol from blood
DNA is an extremely stable molecule, but enzymes which catalyse the breakdown of nucleic acids (nucleases) are found in all cells. In intact cells the DNA is found in the nucleus and thus is protected from the action of nucleases which are abundant in the lysosomes in the cytoplasm. However when cells are lysed, the membranes of the cell compartments are disrupted, allowing nucleases to come in to contact with the DNA. Thus th. Most samples can be directly lysed with proteinase K, eliminating the need for mechanical disruption and reducing hands-on time.
Optimized protocols for specific sample types provide reproducible purification of high-quality DNA for life science, genotyping, and veterinary pathogen research applications. Diego Chacon-Cortes, Lyn R GriffithsGenomics Research Centre, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, QLD, AustraliaAbstract: Deoxyribonucleic acid (DNA) extraction has considerably evolved since it was initially performed back in 189. It is the first step required for many of the available downstream applications used in the field of molecular biology.
Whole blood samples are one of the main sources used to obtain DNA, and there are many different protocols available to perform nucleic acid extraction on such samples. This protocol yields a highly purified DNA preparation from mouse tail biopsies.1. Remove 0.5 mm of tail into polypropylene microfuge tube (do not mince). (The tubes must have tight-fitting caps, so that there are no leaks in st.