Photosynthesis lab paper chromatography
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Continue reading.Open DocumentSeparation of Photosynthetic Pigments by Paper ChromatographyIntroductionChlorophyll is in fact only one pigment in a group of closely relatedpigments commonly found in photosynthesising plants calledphotosynthetic pigments. This can be demonstrated by extracting thepigments from leaves with acetone and separating them by means ofpaper chromatography. Through capillary action these pigments will travel up the paper until the bonds between the water and pigment become so weak that the pigment must break the attraction and leave itself imprinted at a certain height up the paper.
If chlorophyll is boiled or not exposed to light then no photosynthesis will be preformed. Pigments have different masses and also different wavelengths. The chromatography aspect of this lab shows what pigments are in a sample of blue-green algae and spinach. Factors that affect the outcome are the mass of each pigment, and its affinity for the paper used, and h ow soluble the pigment is in the solvent. Photosynthesis is a process in which light is used to make NADPH and ATP in plants.
Watch glasses, 2 1) Label t wo 50-m L beaker s with y our gro up name o r numbe r. Plant Pigments and Photosynthesis Lab Report Background: (Part A)Paper chromatography is a useful technique for separating and identifying pigments and other molecules from cell extracts that contain a complex mixture of molecules. As solvent moves up the paper, it carries along any substances dissolved in it. The more soluble, the further it travels and vice versa. Beta carotene is the most abundant carotene in plants and is carried along near the solvent front since it is very soluble and forms no hydrogen bonds with cellulose.